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IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Western blot testing of human Jurkat cell lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.
SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation of Integrity and Purity of the Antibody.
IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

CD3e Antibody [PC3/188A]

CATALOG NUMBER: 33-806

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Bovine, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Immunogen:
Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
WB: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Optimal dilution of the CD3e antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD3E
Additional Names:
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Ncbi Gene Id Number:
916
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-806

List Size:
100 ug

List Price:

$626.00

Datasheet
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