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Western blot analysis of various lysates using CD3 Rabbit mAbat 1:1900 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): PC-3 | Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CD3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Flow cytometry: 1X10^6 PC-3 cells (negative control,left) and Human PBMC (right) were surface-stained with CD3 Rabbit mAb(2 μg/mL,orange line) or PSFlo 488 Rabbit IgG isotype control,followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Human PBMC (right) were surface-stained with PSFlo 488 Rabbit IgG isotype control or CD3 Rabbit mAb(2 μg/mL,right).
The multiplex IHC analysis on paraffin-embedded Human breast cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD45 Rabbit mAb with TSA-TYR-520 (Green), CD8A Rabbit mAb with TSA-TYR-570 (Red), and CD3E Rabbit mAb with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
The multiplex IHC analysis on paraffin-embedded Human colon tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD45 Rabbit mAb with TSA-TYR-520 (Green), CD8A Rabbit mAb with TSA-TYR-570 (Red), and CD3E Rabbit mAb with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
The multiplex IHC analysis on paraffin-embedded Human tonsil tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD45 Rabbit mAb with TSA-TYR-520 (Green), CD8A Rabbit mAb with TSA-TYR-570 (Red), and CD3E Rabbit mAb with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
Western blot analysis of various lysates using CD3 Rabbit mAbat 1:1900 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): PC-3 | Exposure time: 20s.

CD3E Rabbit Recombinant Antibody (82-856)

CATALOG NUMBER: 82-856

Clonality:
Monoclonal
Tested Applications:
FC, IHC-P, mIHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
FC, IHC-P, mIHC, WB
Application Note:
WB,1:1000-1:5000, IHC-P,1:50-1:200, mIHC,1:50-1:200, FC,1:100 - 1:500
Positive Control 1:
MOLT-4,Jurkat
Predicted Molecular Weight:
23kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CD3E
Additional Names:
T3E; TCRE; IMD18; CD3epsilon; CD3E
Protein Accession Number:
P07766
Ncbi Gene Id Number:
916
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-856

List Size:
100 uL

List Price:

$477.00

Datasheet
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