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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in Hela cell line lysates (35ug/lane).

CD3EAP Antibody

CATALOG NUMBER: 60-819

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD3EAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-81 amino acids from the N-terminal region of human CD3EAP.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD3EAP
Additional Names:
DNA-directed RNA polymerase I subunit RPA34, A345, Antisense to ERCC-1 protein, ASE-1, CD3-epsilon-associated protein, CAST, CD3E-associated protein, RNA polymerase I-associated factor PAF49, CD3EAP, ASE1, CAST, PAF49
Protein Accession Number:
O15446
Ncbi Gene Id Number:
10849
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Background Reference 1:
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Background Reference 2:
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Background Reference 3:
Vangsted, A.J., et al. Haematologica 94(9):1274-1281(2009)
Background Reference 4:
Nissen, K.K., et al. Anticancer Drugs 20(3):174-178(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-819

List Size:
400 ul

List Price:

$495.00

Datasheet
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