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43-654 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-654 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
43-654 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CD4 Antibody

CATALOG NUMBER: 43-654

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KNKEVSVKRVTQDPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Spleen lysates (calculated MW of 31.3kDa according to NP_001181943.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_000607.1) and isoform 2 (NP_001181943.1).
Predicted Molecular Weight:
Approx 30kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD4
Additional Names:
CD4, CD4 molecule, CD4mut, CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Protein Accession Number:
NP_000607.1,NP_001181943.1
Protein Gi Number:
10835167
Ncbi Gene Id Number:
920
Background Reference 1:
Bernstein HB, Plasterer MC, Schiff SE, Kitchen CM, Kitchen S, Zack JA.CD4 expression on activated NK cells: ligation of CD4 induces cytokine expression and cell migration.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-654

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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