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Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD4.
Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD4.

CD4 Antibody [B486A1]

CATALOG NUMBER: 32-133

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot, IHC and ELISA.
Dilution:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CD4 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD4
Additional Names:
CD4mut
Protein Accession Number:
NP_000607.1
Protein Gi Number:
10835167
Ncbi Gene Id Number:
920
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. This integral membrane glycoprotein of approximately 58 kDa contains four external domains (D1 to D4) that show homology to members of the immunoglobulin (Ig) superfamily. It is responsible for the recognition of the MHC class II antigen. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.
Background Reference 1:
M Benkirane J Virol. 1995 November; 69(11): 6898¨C6903.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-133

List Size:
0.1 mg

List Price:

$313.00

Datasheet
Shipping Info

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