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43-414 (0.03ug/ml) staining of U-2 OS lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-414 (0.03ug/ml) staining of U-2 OS lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CD44 Antibody

CATALOG NUMBER: 43-414

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-NRDGTRYVQKGEYRT
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75-82kDa band observed in lysates of cell line U-2 OS (calculated MW of 81.5kDa according to NP_000601.3 and 76.6 according to NP_001001389.1). Recommended concentration: 0.01-0.03ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms 1, 2, 3, 4, 6, 7, 8 (NP_000601.3; NP_001001389.1; NP_001001390.1; NP_001001391.1; NP_001189484.1; NP_001189485.1; NP_001189486.1).
Predicted Molecular Weight:
Approx 75-82kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD44
Additional Names:
CD44, CD44 molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, extracellular matrix receptor III, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, homing function and Indian blood group system, hyaluronate receptor, phagocytic glycoprotein 1
Protein Accession Number:
NP_000601.3,NP_001001389.1,NP_001001390.1,NP_001001391.1,NP_001001392.1,NP_001189484.1,NP_001189485.1,NP_001189486.1
Protein Gi Number:
308153615
Ncbi Gene Id Number:
960
Background Reference 1:
Zhu X, Morales FC, Agarwal NK, Dogruluk T, Gagea M, Georgescu MM.Moesin is a glioma progression marker that induces proliferation and Wnt/β-catenin pathway activation via interaction with CD44.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-414

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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