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Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD44 antibody (SPM544)
Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD44 antibody (SPM544)

CD44 Antibody [SPM544]

CATALOG NUMBER: 34-189

Clonality:
Monoclonal
Tested Applications:
Flow, Functional Assays, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
African Green Monkey, Baboon, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
African Green Monkey, Baboon, Human
Conjugate:
Unconjugated
Tested Applications:
Flow, Functional Assays, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Functional Studies: Order Ab without BSA/azide
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the anti-CD44 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD44
Additional Names:
CD44, CD44 molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglyc
Ncbi Gene Id Number:
960
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-189

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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