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Human peripheral blood lymphocytes were stained with FITC HI100 with relevant isotype control in Red.
Human peripheral blood lymphocytes were stained with FITC HI100 with relevant isotype control in Red.

CD45RA Antibody [HI100] (FITC)

CATALOG NUMBER: 76-457

Clonality:
Monoclonal
Tested Applications:
Flow
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
FITC
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
FITC
Tested Applications:
Flow
Specificity:
The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes.
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Clonality:
Monoclonal
Isotype:
Mouse IgG2b, kappa
Physical State:
Liquid
Buffer:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Storage Conditions:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Ncbi Official Symbol:
PTPRC
Additional Names:
LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180, PTPRC
Ncbi Gene Id Number:
5788
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes. The CD45RA antigen is expressed by naïve and activated T lymphocytes. The HI100 monoclonal antibody is used as a phenotypic marker to discriminate T lymphocytes subsets.
Background Reference 1:
Knapp W(1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.
Background Reference 2:
Barclay, A. N., Brown, M. H., Law, S. A. K. A., McKnight, A. J., Tomlinson, M. G., van der Merwe, P. A. (1997).The leucocyte antigen factsbook. Academic Press.
Background Reference 3:
Yamada, T., Zhu, D., Saxon, A., Zhang, K. (2002). CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase.Journal of Biological Chemistry,277(32), 28830-28835.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

76-457

List Size:
100 tests

List Price:

$253.00

Datasheet
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