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IHC testing of human tonsil (10X) stained with CD45RB antibody (PD7/26).
IHC testing of human tonsil (20X) stained with PD7/26 mAb.
Western blot testing of Daudi cell lysate with CD45RB antibody (clone PD7/26). Predicted molecular weight: ~147/180~220kDa (unmodified/glycosylated).
SDS-PAGE Analysis of Purified, BSA-Free CD45RB Antibody (clone PD7/26). Confirmation of Integrity and Purity of the Antibody.
IHC testing of human tonsil (10X) stained with CD45RB antibody (PD7/26).

CD45RB Antibody [PD7/26]

CATALOG NUMBER: 33-196

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Western Blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G purified
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PTPRC
Additional Names:
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Ncbi Gene Id Number:
5788
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (Cat No V2243).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-196

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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