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Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35kDa but observed at 35-60kDa depending on glycosylation level.
Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35kDa but observed at 35-60kDa depending on glycosylation level.

CD47 Antibody [B6H12.2]

CATALOG NUMBER: 33-099

Clonality:
Monoclonal
Tested Applications:
Blocking, Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
Blocking, Flow, IF, IHC, WB
Application Note:
Functional Activity (Blocking): Order BSA/Azide-free format
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Western blot: 2-5 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD47
Additional Names:
Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6
Ncbi Gene Id Number:
961
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-099

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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