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Western blot analysis of CD49e antibody in mouse bladder tissue lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells using CD49e Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CD49e antibody in mouse bladder tissue lysates (35ug/lane)

CD49e Antibody

CATALOG NUMBER: 61-569

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CD49e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-824 amino acids from the C-terminal region of human CD49e.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
115 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ITGA5
Additional Names:
Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5, FNRA
Protein Accession Number:
P08648
Protein Gi Number:
23831237
Ncbi Gene Id Number:
3678
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD49e belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein is the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Background Reference 1:
Boehmler,A.M., J. Immunol. 182 (11), 6789-6798 (2009)
Background Reference 2:
Okazaki,T., Am. J. Pathol. 174 (6), 2378-2387 (2009)
Background Reference 3:
Schornberg,K.L., Proc. Natl. Acad. Sci. U.S.A. 106 (19), 8003-8008 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-569

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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