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43-225 (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
43-225 (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

CD57 / B3GAT1 Antibody

CATALOG NUMBER: 43-225

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-HVETPRNYKLRGD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 38.3kDa according to NP_061114.2). Recommended concentration: 0.1-0.3ug/ml.An additional band of 17kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Specificity:
Reported variants represent identical protein: NP_473366.1, NP_061114.2.
Predicted Molecular Weight:
Approx 38kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CD57 / B3GAT1
Additional Names:
B3GAT1, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, LEU7 antigen, OTTHUMP00000235432, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, glcUAT-P, glucuronosyltransferase P
Protein Accession Number:
NP_061114.2
Protein Gi Number:
205830910
Ncbi Gene Id Number:
27087
Background Reference 1:
Kähler AK, Djurovic S, Rimol LM, Brown AA, Athanasiu L, Jönsson EG, Hansen T, Gústafsson O, Hall H, Giegling I, Muglia P, Cichon S, Rietschel M, Pietiläinen OP, Peltonen L, Bramon E, Collier D, St Clair D, Sigurdsson E, Petursson H, Rujescu D, Melle I, WCandidate gene analysis of the human natural killer-1 carbohydrate pathway and perineuronal nets in schizophrenia: B3GAT2 is associated with disease risk and cortical surface area.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-225

List Size:
0.1 mg

List Price:

$430.00

Out of stock

Datasheet
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