<b> Immunofluorescence staining of fixed Jurkat cells with anti-CD59 antibody 193-27 (</b> Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric r version of 193-27 (24-760) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-760- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

<b> Flow cytometry using the anti-CD59 antibody 193-27 ( </b> Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of 193-27 (24-760- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Jurkat cells with anti-CD59 antibody 193-27 ( Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric r version of 193-27 (24-760) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-760- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

CD59 Antibody [193-27], Rabbit IgG

CATALOG NUMBER: 24-760

Clonality:

Recombinant Monoclonal

Tested Applications:

Flow, IF, IHC

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Species Reactivity:

Human

Species:

Rabbit

Original Species Name:

Mouse

Chimeric Use Statement:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

Immunogen:

This antibody was raised by immunizng mice with stimulated human leukocytes.

Conjugate:

Unconjugated

Tested Applications:

Flow, IF, IHC

Advanced Validation

Properties

Clonality:

Recombinant Monoclonal

Isotype:

IgG kappa

Buffer:

PBS with 0.02% Proclin 300.

Concentration:

batch dependent

Storage Conditions:

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Additional Info

Ncbi Official Symbol:

CD59

Additional Names:

1F5, 1F5 antigen, CD59 glycoprotein, 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-inhibitory protein, MAC-IP, MEM43 antigen, Membrane attack complex inhibition factor, MACIF, Membrane inhibitor of reactive lysis, MIRL, Protectin

Ncbi Gene Id Number:

966

User Note:

For research use only.

Background

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-760

List Size:

0.2 mg

List Price:

$598.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

Cookie	Duration	Description
cookielawinfo-checbox-analytics	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics".
cookielawinfo-checbox-functional	11 months	The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional".
cookielawinfo-checbox-others	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
cookielawinfo-checkbox-necessary	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary".
cookielawinfo-checkbox-performance	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance".
viewed_cookie_policy	11 months	The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data.

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

CD59 Antibody [193-27], Rabbit IgG

Clonality:

Tested Applications:

Species Reactivity:

Conjugate:

Species Reactivity:

Species:

Original Species Name:

Chimeric Use Statement:

Immunogen:

Conjugate:

Tested Applications:

Clonality:

Isotype:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)

HIV-1 p24 Antibody [9D2F6] (biotin)

HIV-1 p24 Antibody [9D2F6]