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Western blot analysis of CD59 in mouse spleen tissue lysate with CD59 antibody at 1 μg/mL
Western blot analysis of CD59 in mouse spleen tissue lysate with CD59 antibody at 1 μg/mL
Trial Size

CD59 Antibody

CATALOG NUMBER: 7235

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (86%)
Immunogen:
Rabbit polyclonal CD59 antibody was raised against a 14 amino acid peptide near the amino terminus of human CD59.
The immunogen is located within the first 50 amino acids of CD59.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CD59 antibody can be used for detection of CD59 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Predicted Molecular Weight:
14 kDa
Purification:
CD59 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CD59 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CD59 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CD59
Additional Names:
CD59 Antibody: 1F5, EJ16, EJ30, EL32, G344, MIN1, MIN2, MIN3, MIRL, HRF20, MACIF, MEM43, MIC11, MSK21, 16.3A5, HRF-20, MAC-IP, p18-20, CD59 glycoprotein, 1F5 antigen
Protein Accession Number:
NP_000602
Protein Gi Number:
10835165
Ncbi Gene Id Number:
966
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD59 Antibody: The complement regulatory protein CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and ultimately cerebral infarction.
Background Reference 1:
Venneker GT and Asghar SS. CD59: a molecule involved in antigen presentation as well as downregulation of membrane attack complex. Exp. Clin. Immunogenet. 1992; 9:33-47.
Background Reference 2:
Kimberly FC, Sivasankar B, and Paul Morgan B. Alternative roles for CD59. Mol. Immunol. 2007; 44:73-81.
Background Reference 3:
Ninomiya H and Sims PJ. The human complement regulatory protein CD59 binds to the alpha-chain of C8 and to the “b” domain of C9. J. Biol. Chem. 1992; 267:13675-80.
Background Reference 4:
Deckert M, Ticchioni M, Mari B, et al. The glycosylphosphatidylinositol-anchored CD59 protein stimulates both T cell receptor zeta/ZAP-70-dependent and –independent signaling pathways in T cells. Eur. J. Immunol. 1995; 25:1815-22

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7235

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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