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Western blot analysis of lysates from Mouse liver using CD66a/CEACAM1a Rabbit mAb at 1:5000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time:30s.
Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using CD66a/CEACAM1a Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using CD66a/CEACAM1a Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Mouse liver tissue using CD66a/CEACAM1a Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse small intestine tissue using CD66a/CEACAM1a Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse spleen tissue using CD66a/CEACAM1a Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Flow cytometry: 1X10^6 3T3-L1 cells (Low Expression,left) and C57BL/6 mouse Splenocytes (right) were surface-stained with CD66a/CEACAM1a Rabbit mAb or PE Rabbit IgG isotype control, followed by PE conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with PE Rabbit IgG isotype control or CD66a/CEACAM1a Rabbit mAb.
Western blot analysis of lysates from Mouse liver using CD66a/CEACAM1a Rabbit mAb at 1:5000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time:30s.

CD66a/CEACAM1 Rabbit Recombinant Antibody (83-099)

CATALOG NUMBER: 83-099

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF-P, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF-P, IHC-P, WB
Application Note:
WB,1:5000 - 1:20000, IF-P,1:200 - 1:800, IHC-P,1:200 - 1:2000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse liver
Predicted Molecular Weight:
57kDa/50kDa/37kDa/30kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Ceacam1
Additional Names:
Bgp; Cc1; Hv2; Bgp1; Cea1; Cea7; Hv-2; MHVR; bb-1; C-CAM; CD66a; Cea-1; Cea-7; MHVR1; Mhv-1; mCEA1; mmCGM1; mmCGM2; mmCGM1a
Protein Accession Number:
P31809
Ncbi Gene Id Number:
26365
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables several functions, including signaling receptor binding activity; virion binding activity; and virus receptor activity. Involved in several processes, including common myeloid progenitor cell proliferation; granulocyte colony-stimulating factor signaling pathway; and negative regulation of macromolecule metabolic process. Acts upstream of or within several processes, including modulation by host of viral process; regulation of T cell activation; and regulation of intracellular signal transduction. Located in cell projection membrane; external side of plasma membrane; and extracellular space. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; genitourinary system; and skeleton. Orthologous to several human genes including CEACAM1 (CEA cell adhesion molecule 1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-099

List Size:
100 uL

List Price:

$477.00

Datasheet
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