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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

CD69 Antibody

CATALOG NUMBER: 61-918

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD69 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human CD69.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD69
Additional Names:
Early activation antigen CD69, Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69, CD69, CLEC2C
Protein Accession Number:
Q07108
Protein Gi Number:
584906
Ncbi Gene Id Number:
969
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the calcium dependent lectinsuperfamily of type II transmembrane receptors. Expression of theencoded protein is induced upon activation of T lymphocytes, andmay play a role in proliferation. Furthermore, the protein may actto transmit signals in natural killer cells and platelets.Alternative splicing results in multiple transcript variants.
Background Reference 1:
Davila, S., et al. Genes Immun. (2010) In press :
Background Reference 2:
Kolenko, P., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 65 (PT 12), 1258-1260 (2009) :
Background Reference 3:
Hu, M., et al. J. Mol. Recognit. 22(6):516-520(2009)
Background Reference 4:
Radstake, T.R., et al. PLoS ONE(6), E5981 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-918

List Size:
400 ul

List Price:

$495.00

Datasheet
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