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Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysates from U266B1,HepG2 cell line,mouse brain tissue lysate(from left to right), using NT5E-M535 Antibody at 1:2000 at each lane. Lysates at 20ug per lane.
Western blot analysis of NT5E using rabbit polyclonal NT5E Antibody (M535) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analysis of CD73 Pab in mouse brain tissue lysate
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.

CD73 Antibody

CATALOG NUMBER: 60-899

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NT5E
Additional Names:
5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Protein Accession Number:
P21589
Protein Gi Number:
112825
Ncbi Gene Id Number:
4907
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.
Background Reference 1:
Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003). Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002). Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990). Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988). Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-899

List Size:
400 ul

List Price:

$495.00

Datasheet
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