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IHC testing of FFPE human tonsil with CD74 antibody (clone CDLA74-1).
IHC testing of FFPE human tonsil with CD74 antibody (clone CDLA74-1).

CD74 Antibody [CDLA74-1]

CATALOG NUMBER: 34-016

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry: 1-2 ug/ml (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the CD74 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. The CD74 antibody epitope is known to be extracellular.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD74
Additional Names:
CD74, DHLAG, HLADG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invar
Ncbi Gene Id Number:
972
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
CD74 associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [RefSeq]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-016

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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