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<b> Immunofluorescence staining of fixed Daudi cells with anti-CD74 antibody LN-2 (</b> Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of LN-2 (24-766) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-766- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Western Blot using anti-CD74 antibody LN-2 (</b> Daudi cell lysate (A)- human spleen (B) and human tonsil (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of LN-2 (24-766) at 0.0001 ug/ml- 0.001 ug/ml and 0.0001 ug/ml- respectively- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Flow cytometry using the Anti-CD74 antibody LN-2 ( </b> Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of LN-2 (24-766- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed Daudi cells with anti-CD74 antibody LN-2 ( Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of LN-2 (24-766) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-766- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

CD74 Antibody [LN-2], Rabbit IgG

CATALOG NUMBER: 24-766

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC, IP, WB
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human, Mouse, Rat
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunizing mice with SU-DHL-4 lymphoma cells.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, IP, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
CD74
Additional Names:
Ia antigen, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, LN2
Ncbi Gene Id Number:
972
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-766

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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