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Western blot analysis of lysates from Raji cells, using CD79B Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Immunofluorescence analysis of Daudi cells using CD79B Rabbit mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Flow cytometry:1X106 Human PBMC were surface-stained with PSFlo 488 Rabbit anti-Human/Monkey CD19 mAb and Rabbit IgG isotype control or CD79B Rabbit mAb, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Cells in the lymphocyte gate were used for analysis.
Western blot analysis of lysates from Raji cells, using CD79B Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

CD79B Rabbit Recombinant Antibody (82-749)

CATALOG NUMBER: 82-749

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, WB
Application Note:
WB,1:500 - 1:1000, IF/ICC,1:50 - 1:200, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Raji
Predicted Molecular Weight:
14kDa/26kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CD79B
Additional Names:
CD79B; AGM6; B29; IGB; CD79b molecule
Protein Accession Number:
P40259
Ncbi Gene Id Number:
974
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-749

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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