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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD8β, using CD8β Rabbit mAb at 1:80000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.
Western blot analysis of lysates from RPMI 8226 cells using CD8β Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10 s.
Flow cytometry: 1X10^6 293F cells (negative control,left) and 293F (Transfection,right) cells were surface-stained with CD8β Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or CD8β Rabbit mAb.
Flow cytometry: 1X10^6 Human PBMC were surface-stained with CD8β Rabbit mAb or PSFlo 647 Rabbit IgG isotype control. Non-fluorescently stained Human PBMC were used as blank control (red line).
Flow cytometry: 1X10^6 Human PBMC were surface-stained with PSFlo 647 Rabbit IgG isotype control or CD8β Rabbit mAb.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD8β, using CD8β Rabbit mAb at 1:80000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.

CD8β Rabbit Recombinant Antibody (83-007)

CATALOG NUMBER: 83-007

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:10000 - 1:90000, FC,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
24kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CD8B
Additional Names:
LY3; P37; LEU2; LYT3; Ly-3; CD8B1; CD8beta; CD8β
Protein Accession Number:
P10966
Ncbi Gene Id Number:
926
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-007

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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