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Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL. <br><br>Red: CD80 Antibody [11D1] (RF16044) <br> Blue: DAPI staining
Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL. <br><br>Red: CD80 Antibody [11D1] (RF16044) <br> Blue: DAPI staining
Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL. <br><br>Red: CD80 Antibody [11D1] (RF16044) <br> Blue: DAPI staining
Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.
Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.
Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.
Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Trial Size

CD80 Antibody [11D1]

CATALOG NUMBER: RF16044

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
CD80 antibody was raised against the extracellular domain of human CD80.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P
Application Note:
CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.
Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1340 - Human Stomach Carcinoma Tissue Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue
Predicted Molecular Weight:
Predicted: 32 kDa
Observed: 50 kDa
Purification:
CD80 Antibody is supplied as protein A purified IgG1.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD80
Additional Names:
CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
Protein Accession Number:
NP_005182
Protein Gi Number:
4885123
Ncbi Gene Id Number:
941
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Background Reference 1:
Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
Background Reference 2:
Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

RF16044

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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