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<strong> Figure 1 WB Validation in Human Raji Cells </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 2 WB Validation in Human Thymus </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000
<strong>Figure 3 WB Validation in Human Tonsil </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 WB Validation in Rat Thymus </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 8 Immunofluorescence Validation of CD80 in Mouse Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 9 Immunofluorescence Validation of CD80 in Rat Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Flow Cytometry Validation of CD80 in K562 Cells 1X10^6/mL K562 cells were intracellularly stained with 2 μg/mL of CD80 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Figure 1 WB Validation in Human Raji Cells
Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

CD80 Antibody

CATALOG NUMBER: 8679

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (71%)
Immunogen:
Anti-CD80 antibody (8679) was raised against a peptide corresponding to 17 amino acid synthetic peptide near the amino terminus of human CD80.

The immunogen is located within amino acids 60-110 of CD80.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB, IHC-P/IF
Application Note:
WB: 1-4 μg/mL; IHC-P/IF: 5-20 μg/mL; FC: 2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in the human, mouse and rat samples; Flow Cytometry in human samples.. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1315 – Human Tonsil Tissue Lysate
Positive Control 2:
Cat. No. 1314 – Human Thymus Tissue Lysate
Positive Control 3:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 4:
Cat. No. 1471 - Rat Thymus Tissue Lysate
Predicted Molecular Weight:
Predicted: 60 kD

Observed: 60 kD
Purification:
CD80 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD80
Additional Names:
CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
Protein Accession Number:
NP_005182
Protein Gi Number:
4885123
Ncbi Gene Id Number:
941
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Background Reference 1:
Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
Background Reference 2:
Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8679

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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