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<strong> Figure 1 WB Validation in Human Raji Cells </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 2 WB Validation in Human Thymus </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000
<strong>Figure 3 WB Validation in Human Tonsil </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 WB Validation in Rat Thymus </strong><br>Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of CD80 in Human Tonsil</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 8 Immunofluorescence Validation of CD80 in Mouse Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 9 Immunofluorescence Validation of CD80 in Rat Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling CD80 with 8679 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Flow Cytometry Validation of CD80 in K562 Cells 1X10^6/mL K562 cells were intracellularly stained with 2 μg/mL of CD80 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Figure 1 WB Validation in Human Raji Cells
Loading: 15 μg of lysate Antibodies: CD80, 8679, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Green Product
Trial Size

CD80 Antibody

CATALOG NUMBER: 8679

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (71%)
Immunogen:
Anti-CD80 antibody (8679) was raised against a peptide corresponding to 17 amino acid synthetic peptide near the amino terminus of human CD80.

The immunogen is located within amino acids 60-110 of CD80.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB, IHC-P/IF
Application Note:
WB: 1-4 μg/mL; IHC-P/IF: 5-20 μg/mL; FC: 2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in the human, mouse and rat samples; Flow Cytometry in human samples.. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1315 – Human Tonsil Tissue Lysate
Positive Control 2:
Cat. No. 1314 – Human Thymus Tissue Lysate
Positive Control 3:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 4:
Cat. No. 1471 - Rat Thymus Tissue Lysate
Predicted Molecular Weight:
Predicted: 60 kD

Observed: 60 kD
Purification:
CD80 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
CD80 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CD80 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD80
Additional Names:
CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
Protein Accession Number:
NP_005182
Protein Gi Number:
4885123
Ncbi Gene Id Number:
941
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Background Reference 1:
Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
Background Reference 2:
Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8679

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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