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Western blot analysis of CD81 in human lung tissue lysate with CD81 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking petide.
Immunohistochemistry of CD81 in human lung tissue with CD81 antibody at 5 μg/mL.
Immunofluorescence of CD81 in A549 cells with CD81 antibody at 20 μg/mL.
Immunohistochemistry of CD81 in human brain tissue with CD81 antibody at 2 μg/ml.
Immunofluorescence of CD81 in human brain tissue with CD81 antibody at 10 μg/ml.<br><br>Green: CD81 Antibody (5195) <br> Blue: DAPI staining
Western blot analysis of CD81 in human lung tissue lysate with CD81 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking petide.
Cell Treatment
Trial Size

CD81 Antibody

CATALOG NUMBER: 5195

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81.
The immunogen is located within amino acids 30 - 80 of CD81.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CD81 antibody can be used for detection of CD81 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1283 - RAW 264.7 Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3:
Cat. No. 17-003 - A549 Cell Slide
Predicted Molecular Weight:
Predicted: 26 kDa
Observed: 25 kDa
Purification:
CD81 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CD81 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CD81 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD81
Additional Names:
CD81 Antibody: S5.7, CVID6, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Tspan-28
Protein Accession Number:
NP_004347
Protein Gi Number:
4757944
Ncbi Gene Id Number:
975
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD81 Antibody: CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, a group of cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that associates with integrins and appears to promote muscle cell fusion and support myotube maintenance. Along with SCARB1, LDL-R, and CLDN1, CD81 has been reported to be an entry factor for the Hepatitis C virus. Finally, recent evidence indicates that the CD81 gene is localized in a tumor-suppressor gene region and is thus a candidate gene for malignancies.
Background Reference 1:
Oren R, Takahashi S, Doss C, et al. TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol. Cell Biol. 1990; 10:4007-15.
Background Reference 2:
Charrin S, le Naour F, Silvie O, et al. Lateral organization of membrane proteins: tetraspanins spin their web. Biochem. J. 2009; 420:133-54.
Background Reference 3:
Mannion BA, Berditchevski F, Kraeft SK, et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J. Immunol. 1996; 157:2039-47.
Background Reference 4:
Tacvhibana I and Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J. Cell Biol. 1999; 146:893-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5195

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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