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Western blot analysis of extracts of various cell lines, using CD9 antibody (13-804).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse spleen using CD9 antibody (13-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CD9 antibody (13-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using CD9 antibody (13-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human spleen using CD9 antibody (13-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CD9 antibody (13-804) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of CRC cells using CD9 antibody (13-804).
Immunofluorescence analysis of SW480 cells using CD9 antibody (13-804).
Western blot analysis of extracts of various cell lines, using CD9 antibody (13-804).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

CD9 Antibody [4F10B5]

CATALOG NUMBER: 13-804

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human CD9
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
Mouse heart
Positive Control 2:
Jurkat
Predicted Molecular Weight:
Observed: 25kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CD9
Additional Names:
CD9, 5H9 antigen, BA2, BA-2/p24 antigen, CD9 antigen, CD9 antigen (p24), DRAP-27, MIC3, MRP-1, Leukocyte antigen MIC3, Tetraspanin-29, TSPAN29, BTCC-1, CD9 molecule, Motility related protein-1, Motility-related protein, TSPAN-29
Ncbi Gene Id Number:
928
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-804

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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