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Western blot analysis of CD9 in (A) human ovary, (B) human uterus, (C) human ovary tumor, and (D) human uterus tumor tissue lysate with CD9 antibody at 1 μg/ml.
Western blot analysis of CD9 in (A) human ovary, (B) human uterus, (C) human ovary tumor, and (D) human uterus tumor tissue lysate with CD9 antibody at 1 μg/ml.
Trial Size

CD9 Antibody

CATALOG NUMBER: 8531

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Cat: (90%), Bovine: (89%), Mouse: (84%), Rat: (84%), Boar: (78%)
Immunogen:
CD9 antibody was raised against a peptide corresponding to 19 amino acids near the center of human CD9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CD9 antibody can be used for Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1384 - Human Uterus Tissue Lysate
Predicted Molecular Weight:
Predicted: 25 kDa
Observed: 25 kDa
Purification:
CD9 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CD9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CD9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD9
Additional Names:
CD9 Antibody: CD9 molecule, MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29, TSPAN-29
Protein Accession Number:
NP_001760
Protein Gi Number:
4502693
Ncbi Gene Id Number:
928
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD9 Antibody: CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Like other tetraspanins, CD9 is a cell surface glycoproteins play a role in many cellular processes including differentiation, adhesion, and signal transduction (1). CD9 expression plays a critical role in the suppression of cancer cell motility and metastasis. In one study, the knockdown of CD9 expression suppressed the metastatic capacity of human breast cancer cells (2), while other results have shown the opposite effect, suggesting that different proteins associated with CD9 account for its abilities to promote or suppress metastasis (3).
Background Reference 1:
Murayama Y, Oritani K, and Tsutsui S. Novel CD9-targeted therapies in gastric cancer. World J. Gastroenterol. 2015; 21:3206-13.
Background Reference 2:
Rappa G, Green TM, Karbanova J, Corbeil D, et al. Tetraspanin CD9 determines invasiveness and tumorigenicity of human breast cancer cells. Oncotarget 2015; 6:7970-91.
Background Reference 3:
Zoller M. Tetraspanins: push and pull in suppressing and promoting metastasis. Nat. Rev. Cancer 2009; 9:40-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8531

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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