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Western blot analysis of various lysates, using CD96 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Flow cytometry: 1X10^6 HeLa cells (negative control,left) and MOLT-4 (right) cells were surface-stained with Human CD96 mAb or PSFlo 594 Rabbit IgG isotype control, followed by PSFlo 594 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PSFlo 594 Rabbit IgG isotype control or Human CD96 mAb.
Western blot analysis of various lysates, using CD96 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

CD96 Rabbit Recombinant Antibody (82-809)

CATALOG NUMBER: 82-809

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:500 - 1:1000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Jurkat
Predicted Molecular Weight:
65kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CD96
Additional Names:
Cell surface antigen CD96; CD96
Protein Accession Number:
P40200
Ncbi Gene Id Number:
10225
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD96 (cluster of differentiation 96) belongs to the IgG (immunoglobulin) superfamily receptors.It is expressed in the spinal cord, kidney, trachea, digestive tissues, prostate, placenta, bone of adults and fetal brain and liver. This gene is located on human chromosome 3q13.The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-809

List Size:
100 uL

List Price:

$477.00

Datasheet
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