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43-210 (0.3ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-210 (1ug/ml) staining of Mouse Colon (wt, left lane, knock-out right lane) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by infrared fluorescence (Odyssey). Data obtained from anonymous customer
43-210 (0.3ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cd97 Antibody

CATALOG NUMBER: 43-210

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
The immunogen for this antibody is: C-TSQTRALRSSES
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in Mouse Thymus and Spleen lysates (calculated MW of 80.2kDa according to NP_001156503.1). The beta chain is detected as a ~30kDa band in Mouse Colon (transgenic overexpressing Cd76 in intestines) and not in the knock-out. Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_036055.2; NP_001156501.1; NP_001156502.1; NP_001156503.1).
Predicted Molecular Weight:
Approx 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Cd97
Additional Names:
Cd97, CD97 antigen, AA409984, TM7LN1, EGF-TM7 receptor, adhesion G protein-coupled receptor E5
Protein Accession Number:
NP_036055.2,NP_001156501.1,NP_001156502.1,NP_001156503.1
Protein Gi Number:
251823835
Ncbi Gene Id Number:
26364
Background Reference 1:
Hoek RM, de Launay D, Kop EN, Yilmaz-Elis AS, Lin F, Reedquist KA, Verbeek JS, Medof ME, Tak PP, Hamann J.Deletion of either CD55 or CD97 ameliorates arthritis in mouse models.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-210

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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