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IHC testing of FFPE human Ewing's sarcoma with CD99 antibody. Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
IHC testing of FFPE human Ewing's sarcoma with CD99 antibody. Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

CD99 Antibody

CATALOG NUMBER: 33-638

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human protein was used as the immunogen for the CD99 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 2-4 ug/million cells in 0.1ml
Immunofluorescence: 2-4 ug/ml
Immunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RT
Titering of the CD99 antibody may be required for optimal performance.
Purification:
Protein A affinity chromatography
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD99
Additional Names:
CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 molecule, E2 antigen, HBA71
Ncbi Gene Id Number:
4267
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-638

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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