Western blot analysis of extracts of various cell lines, using CDA antibody (15-295) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human liver cancer using CDA Antibody (15-295) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using CDA Antibody (15-295) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using CDA Antibody (15-295) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using CDA Antibody (15-295) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using CDA Antibody (15-295) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using CDA Antibody (15-295) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CDA antibody (15-295) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

CDA Antibody

CATALOG NUMBER: 15-295

Clonality:

Polyclonal

Tested Applications:

IF, IHC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human CDA (NP_001776.1).

Conjugate:

Unconjugated

Tested Applications:

IF, IHC, WB

Application Note:

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100

Positive Control 1:

HeLa

Positive Control 2:

HepG2

Positive Control 3:

293T

Positive Control 4:

Jurkat

Positive Control 5:

SW620

Positive Control 6:

Mouse kidney

Predicted Molecular Weight:

Observed: 15kDa,35kDa,65kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

CDA

Additional Names:

CDA, Cytidine aminohydrolase, Cytidine deaminase, Small cytidine deaminase, CDD, Cytosine nucleoside deaminase

Ncbi Gene Id Number:

978

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-295

List Size:

100 uL

List Price:

$436.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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CDA Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Positive Control 4:

Positive Control 5:

Positive Control 6:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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