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Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstratesdetected the cdc14 protein (arrow).
Cdc14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstratesdetected the cdc14 protein (arrow).

Cdc14 Antibody

CATALOG NUMBER: 63-472

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Cdc14 antibody is generated from rabbits immunized with a recombinant protein encoding aa 1~379 of human cdc14.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
67 kDa
Purification:
This antibody is purified through a protein A column, followed by dialysis against PBS
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC14A
Additional Names:
Dual specificity protein phosphatase CDC14A, CDC14 cell division cycle 14 homolog A, CDC14A
Protein Accession Number:
Q9UNH5
Protein Gi Number:
55976620
Ncbi Gene Id Number:
8556
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms.
Background Reference 1:
Kaiser,B.K., et al. Mol. Biol. Cell 13 (7), 2289-2300 (2002)
Background Reference 2:
Mailand,N., et al. Nat. Cell Biol.(4), 317-322 (2002)
Background Reference 3:
Bembenek,J. and Yu,H. J. Biol. Chem. 276 (51), 48237-48242 (2001)
Background Reference 4:
Li,L., et al. J. Biol. Chem. 275 (4), 2410-2414 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-472

List Size:
400 ul

List Price:

$495.00

Datasheet
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