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IHC testing of formalin-fixed, paraffin-embedded human tonsil with Cdc2 antibody (CDK1/873).
IHC testing of formalin-fixed, paraffin-embedded human tonsil with Cdc2 antibody (CDK1/873).

Cdc2 Antibody [CDK1/873]

CATALOG NUMBER: 33-513

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Mink, Monkey
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Mink, Monkey
Immunogen:
Recombinant human protein was used as the immunogen for the Cdc2 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)
Optimal dilution of the Cdc2 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Specificity:
Does not react with Mouse, Rat, Xenopus, Drosophila, S. pombe, S. cerevisiae
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CDK1
Additional Names:
Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2
Ncbi Gene Id Number:
983
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-513

List Size:
100 ug

List Price:

$626.00

Datasheet
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