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Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 (Phospho-Thr161).
Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161).

CDC2 (phospho Thr161) Antibody

CATALOG NUMBER: 79-166

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CDC2 (Phospho-Thr161) antibody was raised against a peptide sequence around phosphorylation site of threonine161 (T-Y-T (p) -H-E) derived from Human CDC2.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of CDC2 only when phosphorylated at threonine 161.
Predicted Molecular Weight:
34 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDK1
Additional Names:
CDC2, CDC28A, P34CDC2, C1orf215, LAP18, OP18, CDC28, CDC2A, CDK1, Cyclin-dependent kinase 1
Protein Accession Number:
NP_001163877.1
Protein Gi Number:
281427278
Ncbi Gene Id Number:
983
Background:
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Background Reference 1:
Ukomadu C, et al. (2003) J Biol Chem; 278 (7): 4840-6. Morris MC, et al. (2002) J Biol Chem; 277 (26): 23847-53. Brown NR, et al. (1999) J Biol Chem; 274 (13): 8746-56. Liu Y, et al. (2004) J Biol Chem; 279 (6): 4507-14.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-166

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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