Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot of human T47D cells showing phospho-specific immunolabeling of the ~34k cdc2 protein phosphorylated at Tyr15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr15.
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~34k cdc2 protein phosphorylated at Tyr15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr15.

Cdc2 (phospho Tyr15) Antibody

CATALOG NUMBER: XPS-1003

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Dog, Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Frog, Human, Mouse, Rat
Immunogen:
Cdc2 (Tyr15) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human, mouse, rat and xenopus cdc2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody. Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) has not been determined. Drosophila, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
Cdc2 antibody is specific for cdc2 phosphorylated at Tyr15.
Predicted Molecular Weight:
34
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Clk2
Protein Accession Number:
Q5XI98
Protein Gi Number:
81910362
Ncbi Gene Id Number:
365842
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2.
Background Reference 1:
Sible, J.C., "Cell biology: thanks for the memory," Nature (London), 426 (2003) 392-393.
Background Reference 2:
Sagata, N., "Molecular biology: untangling checkpoints," Science, 298 (2002) 1905-1907.
Background Reference 3:
Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K., and Fukui, M., "Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines," J. Neurooncol., 59 (2002) 7-13.
Background Reference 4:
Maller, J.L., "Mitotic control," Curr. Opinion. Cell Biology, 3 (1991) 269-275.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1003

List Size:
0.1 mL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369