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IHC testing of FFPE gastric carcinoma with Cdc20 antibody (clone AR12)
SDS-PAGE Analysis of Purified, BSA-Free Cdc20 Antibody (clone AR12). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE gastric carcinoma with Cdc20 antibody (clone AR12)

Cdc20 Antibody [AR12]

CATALOG NUMBER: 33-106

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Denatured recombinant human protein was used as the immunogen for this Cdc20 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdc20 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G purified antibody
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CDC20
Additional Names:
Cell division cycle protein 20 homolog, p55CDC, CDC20
Ncbi Gene Id Number:
991
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 kinase (p34Cdc2) exhibits kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-106

List Size:
100 ug

List Price:

$626.00

Datasheet
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