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Western blot analysis of lysed extracts from HeLa and 3T6 cells using cdc25A (Ab-76).
Western blot analysis of lysed extracts from HeLa and 3T6 cells using cdc25A (Ab-76).

cdc25A (Ab 76) Antibody

CATALOG NUMBER: 79-562

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
cdc25A (Ab-76) antibody was raised against a peptide sequence around aa. 73~77 (M-G-S-S-E) derived from Human cdc25A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total cdc25A protein.
Predicted Molecular Weight:
70 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDC25A
Additional Names:
CDC25A2, CDC25M3, MPIP1
Protein Accession Number:
NP_001780.2
Protein Gi Number:
42490758
Ncbi Gene Id Number:
993
Background:
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Background Reference 1:
Jin J, et al. (2003) Genes 17 (24): 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14): 3694-703.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-562

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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