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Western blot analysis of extracts of various cell lines, using CDC25A antibody (14-120) at 1:800 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using CDC25A antibody (14-120) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using CDC25A antibody (14-120) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibody (14-120) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CDC25A Polyclonal Antibody (14-120) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CDC25A antibody (14-120) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CDC25A Antibody

CATALOG NUMBER: 14-120

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 235-484 of human CDC25A (NP_963861.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
K562
Predicted Molecular Weight:
Observed: 59kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDC25A
Additional Names:
CDC25A, CDC25A2-CAG isoform, Cell division cycle 25A, CDC25A phosphatase, CDC25Hu1, M-phase inducer phosphatase 1, CDC25A2
Ncbi Gene Id Number:
993
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-120

List Size:
100 uL

List Price:

$436.00

Datasheet
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