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Western blot analysis of Cdc25A Pab in mouse kidney tissue lysates (35ug/lane)
Western blot analysis of Cdc25A Pab in mouse kidney tissue lysates (35ug/lane)

Cdc25A Antibody

CATALOG NUMBER: 62-210

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Cdc25A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC25A
Additional Names:
M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A
Protein Accession Number:
P30304
Protein Gi Number:
50403734
Ncbi Gene Id Number:
993
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.
Background Reference 1:
Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004).
Background Reference 2:
Nemoto, K., et al., Prostate 58(1):95-102 (2004).
Background Reference 3:
Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003).
Background Reference 4:
Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-210

List Size:
400 ul

List Price:

$495.00

Datasheet
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