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Western blot analysis of extracts from A549 cells treated with UV using Phospho-CDC25A (Ser178) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from A549 cells treated with UV using Phospho-CDC25A (Ser178) Antibody. The lane on the right is treated with the antigen-specific peptide.

CDC25A (phospho Ser178) Antibody

CATALOG NUMBER: 79-998

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CDC25A (phospho Ser178) antibody was raised against a peptide sequence around phosphorylation site of serine 178 (Q-N-S (p)-A-P) derived from Human CDC25A .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of CDC25A only when phosphorylated at serine 178.
Predicted Molecular Weight:
59 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDC25A
Additional Names:
CDC25M3, MPIP1, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1,
Protein Accession Number:
NP_001780.2
Protein Gi Number:
42490758
Ncbi Gene Id Number:
993
Background:
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Background Reference 1:
Chen MS, Ryan CE, Piwnica-Worms H (2003)Mol Cell Biol 23, 7488-97
Background Reference 2:
Hassepass I, Voit R, Hoffmann I (2003) J Biol Chem 278, 29824-9
Background Reference 3:
Sørensen CS, et al. (2003) Cancer Cell 3, 247-58

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-998

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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