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Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).

cdc25A (phospho Ser76) Antibody

CATALOG NUMBER: 79-169

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
Predicted Molecular Weight:
70 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDC25A
Additional Names:
CDC25A2, MEK1, PRKMK1, CDC25M3, MPIP1
Protein Accession Number:
NP_001780.2
Protein Gi Number:
42490758
Ncbi Gene Id Number:
993
Background:
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Background Reference 1:
Jin J, et al. (2003) Genes 17 (24): 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14): 3694-703.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-169

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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