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Western Blot in Ramos cell line lysates (35ug/lane). CDC25B(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDC25B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot in Ramos cell line lysates (35ug/lane). CDC25B(arrow) was detected using the purified Pab.

CDC25B Antibody

CATALOG NUMBER: 62-765

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CDC25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the Central region of human CDC25B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC25B
Additional Names:
M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2
Protein Accession Number:
P30305
Protein Gi Number:
21264471
Ncbi Gene Id Number:
994
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.
Background Reference 1:
Uchida, S., et al., Biochem. Biophys. Res. Commun. 316(1):226-232 (2004).
Background Reference 2:
Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004).
Background Reference 3:
Wu, W., et al., Cancer Res. 63(19):6195-6199 (2003).
Background Reference 4:
Mils, V., et al., Exp. Cell Res. 285(1):99-106 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-765

List Size:
400 ul

List Price:

$495.00

Datasheet
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