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Western blot analysis of lysed extracts from 3T3 cells untreated (Lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149).
Western blot analysis of lysed extracts from 3T3 cells untreated (Lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149).

Cdc25B (phospho Ser149) Antibody

CATALOG NUMBER: 79-514

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Cdc25B (Phospho-Ser149) antibody was raised against a peptide sequence around phosphorylation site of Serine 149 (F-R-S (p) -L-P) derived from Mouse Cdc25B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
Predicted Molecular Weight:
62 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
Cdc25b
Additional Names:
AI604853, CDC25B
Protein Accession Number:
NP_001104545.1
Protein Gi Number:
161621261
Ncbi Gene Id Number:
12531
Background:
Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
Background Reference 1:
Li G, et al. (2010) Retrovirology. 7:59. Wang Z, et al. (2010) BMC Cancer. 10:233. Lucci MA, et al. (2010) Cell Oncol. 32 (5-6): 361-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-514

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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