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Western blot analysis of CDC25C Antibody in NIH-3T3 cell line lysates (35ug/lane)
Western blot analysis of CDC25C Antibody in NIH-3T3 cell line lysates (35ug/lane)

CDC25C Antibody

CATALOG NUMBER: 63-249

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CDC25C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-142 amino acids from the Central region of human CDC25C.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC25C
Additional Names:
M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C
Protein Accession Number:
P30307
Protein Gi Number:
116242631
Ncbi Gene Id Number:
995
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDC25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest.
Background Reference 1:
Varmeh,S., Mol. Cancer Ther.(12), 3789-3799 (2008)
Background Reference 2:
Long,J.M., Cell Cycle(19), 3062-3073 (2008)
Background Reference 3:
Bonnet,J., Cell Cycle(13), 1991-1998 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-249

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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