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Western blot analysis of extracts of various cell lines, using CDC25C antibody (13-481) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using CDC25C antibody (13-481) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CDC25C Antibody [8C9A9]

CATALOG NUMBER: 13-481

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant protein of human CDC25C
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
K-562
Positive Control 3:
MCF7
Positive Control 4:
Raji
Positive Control 5:
SW480
Positive Control 6:
A375
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
cdc25C
Additional Names:
CDC25C, CDC25, PPP1R60
Ncbi Gene Id Number:
995
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-481

List Size:
100 uL

List Price:

$365.00

Out of stock

Datasheet
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