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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using cdc25C (Phospho-Ser216).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using cdc25C (Phospho-Ser216).
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using cdc25C (Phospho-Ser216).

cdc25C (phospho Ser216) Antibody

CATALOG NUMBER: 79-155

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
cdc25C (Phospho-Ser216) antibody was raised against a peptide sequence around phosphorylation site of serine 216 (S-P-S (p) -M-P) derived from Human cdc25C.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDC25C
Additional Names:
CDC25, PPP1R60, MACS, PRKCSL, CDC25M1, MPIP3
Protein Accession Number:
NP_001781.2
Protein Gi Number:
125625350
Ncbi Gene Id Number:
995
Background:
cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Background Reference 1:
Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3 (4): 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25 (7): 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4 (7): 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99 (4): 1990-1995.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-155

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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