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Western blot analysis of CDC2L6 Antibody in mouse heart tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDC2L6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CDC2L6 Antibody in mouse heart tissue lysates (35ug/lane)

CDC2L6 Antibody

CATALOG NUMBER: 63-774

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CDC2L6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-285 amino acids from the Central region of human CDC2L6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDK19
Additional Names:
Cyclin-dependent kinase 19, CDC2-related protein kinase 6, Cell division cycle 2-like protein kinase 6, Cell division protein kinase 19, Cyclin-dependent kinase 11, Death-preventing kinase, CDK19, CDC2L6, CDK11, KIAA1028
Protein Accession Number:
Q9BWU1
Protein Gi Number:
60391917
Ncbi Gene Id Number:
23097
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDC2L6 is one of the components of the Mediator coactivator complex. The Mediator complex is a multiprotein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex.
Background Reference 1:
Malumbres,M., et.al. Nat. Cell Biol. 11 (11), 1275-1276 (2009)
Background Reference 2:
Loyer,P., et.al., J. Biol. Chem. 283 (12), 7721-7732 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-774

List Size:
400 ul

List Price:

$495.00

Datasheet
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