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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of CDC37 Antibody in MCF-7, A2058 cell line lysates (35ug/lane)
CDC37 Antibody IHC analysis in formalin fixed and paraffin embedded skin followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.

CDC37 Antibody

CATALOG NUMBER: 63-723

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
This CDC37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the Central region of human CDC37.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC37
Additional Names:
Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit, p50Cdc37, Hsp90 co-chaperone Cdc37, N-terminally processed, CDC37, CDC37A
Protein Accession Number:
Q16543
Protein Gi Number:
21542000
Ncbi Gene Id Number:
11140
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDC37 is a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Background Reference 1:
Dai,K., et.al., J. Biol. Chem. 271 (36), 22030-22034 (1996)
Background Reference 2:
Lamphere,L., et.al., Oncogene 14 (16), 1999-2004 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-723

List Size:
400 ul

List Price:

$495.00

Datasheet
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