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45-384 (3.8ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-384 (3.8ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Cdc42-binding kinase alpha Antibody

CATALOG NUMBER: 45-384

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: SGEVRLRQLEQFI-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 30kDa and 40kDa bands in Human Pancreas lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 186kDa according to NP_055641.3 and 196kDa according to NP_003598.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows cytoplasm staining in purkinje cells. Recommended concentration, 3-6ug/ml.
Specificity:
This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CDC42BPA
Additional Names:
CDC42BPA, MRCK, MRCKA, PK428, FLJ23347, KIAA0451, CDC42 binding protein kinase alpha (DMPK-like), ser-thr protein kinase PK428, myotonic dystrophy kinase-related CDC42-binding protein kinase alpha, Ser-Thr protein kinase related to the myotonic dystrophy protein kinase, DKFZp686L1738, CDC42 binding protein kinase beta
Protein Accession Number:
NP_055641.3,NP_003598.2
Protein Gi Number:
30089960
Ncbi Gene Id Number:
8476
Background Reference 1:
Zhao Y, Loyer P, Li H, Valentine V, Kidd V, Kraft AS. Cloning and chromosomal location of a novel member of the myotonic dystrophy family of protein kinases. J Biol Chem. 1997 Apr 11;272(15):10013-20.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-384

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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