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Western Blot at 1:1000 dilution Lane 1: HUVEC whole cell lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: HUVEC whole cell lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.

CDC42EP1 Antibody

CATALOG NUMBER: 60-263

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CDC42EP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-343 amino acids from the C-terminal region of human CDC42EP1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC42EP1
Additional Names:
Cdc42 effector protein 1, Binder of Rho GTPases 5, Serum protein MSE55, CDC42EP1, BORG5, CEP1, MSE55
Protein Accession Number:
Q00587
Protein Gi Number:
462623
Ncbi Gene Id Number:
11135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDC42 is a member of the Rho GTPase family that regulatesmultiple cellular activities, including actin polymerization. Theprotein encoded by this gene is a CDC42 binding protein thatmediates actin cytoskeleton reorganization at the plasma membrane.This protein is secreted and is primarily found in bone marrow.
Background Reference 1:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 2:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Zhang, J., et al. Mol. Biol. Cell 16(2):824-834(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-263

List Size:
400 ul

List Price:

$495.00

Datasheet
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