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Western blot analysis of CDC5L Antibody in MCF-7 cell line lysates (35ug/lane)
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CDC5L Antibody in MCF-7 cell line lysates (35ug/lane)

CDC5L Antibody

CATALOG NUMBER: 63-716

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CDC5L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-213 amino acids from the Central region of human CDC5L.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
92 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC5L
Additional Names:
Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein, CDC5L, KIAA0432, PCDC5RP
Protein Accession Number:
Q99459
Protein Gi Number:
73619933
Ncbi Gene Id Number:
988
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein is a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
Background Reference 1:
Grillari,J., et.al., J. Biol. Chem. 284 (42), 29193-29204 (2009) Zhang,N., et.al., EMBO Rep. 10 (9), 1029-1035 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-716

List Size:
400 ul

List Price:

$495.00

Datasheet
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